The players involved in the incident and the Penguin Football Club did not respond The ages request for comment.
AFL Tasmania regretted the behavior of the players.
"It is not acceptable behavior and we disapprove of it", it said in a statement.
"We will work with the competition and the club to get more information and to see how we can help provide education to the players."
Blackface is wearing make-up and costumes by non-black people.
The practice arose in American & # 39; minstrel shows & # 39; in the 19th century and played a key role in creating racist stereotypes about black people.
In Australia it has repeatedly received strong praise from the Aboriginal community, civil rights and anti-discrimination groups, who claim that it perpetuates or reinforces the humiliating stereotypes.
Last week, Herald Sun cartoonist Mark Knight caused an international storm with his image of Serena Williams who threw a tantrum during the final of the US Open last weekend.
Knight published his response to Saturday's US Open Women's Final – and some have seen that Williams made caricature in a racially surprising way.
Blackface controversies have surfaced quite often in Australia in recent years. In 2016, opal basketball team member Alice Kunek apologized after posting photo's of himself in Blackface.
A picture of Kunek, dressed as rapper Kanye West for the "Silly Sunday" celebrations of the Melbourne Boomers, was placed on social media, which caused an angry reaction from the audience, including Opals star Liz Cambage.
A mother who blackened the skin of her son for a school walk in the book week, also caused viral outrage on social media in 2016 for a moment she described as a "parenting profit".
In her post, shared on social media by furious parents, the mother said that her son had wanted to resemble his "idol", AFL footballer Nic Naitanui.
The Frankston Bombers also apologized after posting photos of teammates in Blackface.
The football and korfball club were taken under fire after posting photos of players dressed for an iPod Shuffle night, including some who blackened their skin color as American rappers. to appear.
In 2011, Qantas had to apologize after having encouraged two men to dress in Blackface as Fijian-born Wallaby Radike Samo during a Bledisloe Cup match in Brisbane.
And in 2013, Cricket Australia had to apologize after filming an image of fans dressed like Indians with beards in colorful lycra, similar to the English left-handed spinner Monty Panesar.
Melissa Cunningham reports breaking news for The Age.